Expression of recombinant human Interleukin 2 (rhIL-2) in E. coli

Expression of recombinant human Interleukin 2 (rhIL-2) in E. coli

Background: interleukin-2 (IL-2) is a protein consisted of 132 amino acids (MW = 1.53 kDa). Aldesleukin is the synthetic form of the protein which is used as an effective function for immuno system. Current study was aimed to investigate the expression of rhIL-2 in E. coli BL21 (DE3) expression system in order to produce an active recombinant form of the protein. Methods: Firstly codon optimization was done for hIL-2 gene. Then the gene was synthesized and inserted in pET-22a vector by a ...

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